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HomeFAQsCan HiFi Taq DNA Ligase be used for cloning?
FAQ: Can HiFi Taq DNA Ligase be used for cloning?
No, HiFi Taq DNA Ligase will not ligate all types of ends. We recommend using T4 DNA Ligase (NEB #M0202) for cloning.
High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.
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