FAQ: What is the smallest 5´ DNA flap Thermostable FEN1 will cleave?

Thermostable FEN1 can cleave a 1 nt DNA flap, but a 3´ 1 nt flap must also be present. Without the presence of the 3´ 1 nt flap, Thermostable FEN1 will not cleave a 1 nt flap.