HomeFAQsWhat is the difference between Rapid PNGase F (non-reducing format) and Endo S (Endo S, NEB# P0741)?
FAQ: What is the difference between Rapid PNGase F (non-reducing format) and Endo S (Endo S, NEB# P0741)?
Rapid PNGase F (non-reducing format) cleaves N-glycans from antibodies between the innermost GlcNAc and asparagine residues. Endo S is an endoglycosidase that cleaves within the chitobiose core of N-linked glycans from the heavy chain of native IgG. With Endo S, a single GlcNAc, with a fucose residue if present, remains attached to the asparagine residue of the IgG chain.
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