FAQ: What types of DNA damage does Tma Endonuclease III act upon?

Tma Endonuclease III acts both as a N-glycosylase and AP-lyase. Tma Endonuclease III recognizes and through its N-glycosylase activity removes oxidized pyrimidine bases from DNA, such as 5,6 dihydroxythymine and thymine glycol (2). The resulting abasic site is then cleaved by the AP-lyase activity. The AP-lyase activity of Tma endonuclease III cleaves the phosphodiester bond 3’ to an abasic site leaving a 3’-α, β-unsaturated aldehyde and a 5’-phosphate.