HomeFAQsWhy do I see a smear above my plasmid band on an agarose gel?
FAQ: Why do I see a smear above my plasmid band on an agarose gel?
The presence of a smear at a higher molecular weight than the plasmid band may indicate genomic DNA is contaminating the prep. While not common, genomic DNA can be sheared during lysis and co-purify with the plasmid. Vigorous shaking or vortexing after addition of buffer B2 will produce sheared genomic DNA and should be avoided. If the lysate is very viscous and does not mix well, use less cells for the miniprep.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.