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HomeFAQsWhat should I do differently when using a low copy plasmid?
FAQ: What should I do differently when using a low copy plasmid?
Recovery of low-copy plasmids is always reduced in comparison to high copy plasmids. Ensuring the culture is grown with proper aeration and harvested before significant cell lysis occurs will increase the number of intact cells harboring the plasmid. Using more cells can make up some of the deficiency, but stay within the supplied guidelines to ensure alkaline lysis is efficient and the column does not get overloaded with cellular components. Please also review our guidelines for working with low copy plasmids and our application note on removal of genomic DNA when working with low copy plasmids.
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