HomeFAQsWhat should I do differently when using a low copy plasmid?
FAQ: What should I do differently when using a low copy plasmid?
Recovery of low-copy plasmids is always reduced in comparison to high copy plasmids. Ensuring the culture is grown with proper aeration and harvested before significant cell lysis occurs will increase the number of intact cells harboring the plasmid. Using more cells can make up some of the deficiency, but stay within the supplied guidelines to ensure alkaline lysis is efficient and the column does not get overloaded with cellular components.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.