HomeFAQsWhat is the smallest volume of elution buffer that can be used with the Monarch Plasmid Miniprep Kit?
FAQ: What is the smallest volume of elution buffer that can be used with the Monarch Plasmid Miniprep Kit?
The protocol is optimized to provide good yields with 30 μl of elution buffer. Lower amounts can be used but overall yield will be reduced due to incomplete wetting of the membrane. Eluting with small volumes such as 10 μl will provide highly concentrated plasmid DNA, but recovery may only reach 60%. Higher elution volumes can also be used, but the eluted DNA will have a lower concentration.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.