HomeFAQsWhat is the smallest volume of elution buffer that can be used with the Monarch Plasmid Miniprep Kit?
FAQ: What is the smallest volume of elution buffer that can be used with the Monarch Plasmid Miniprep Kit?
The protocol is optimized to provide good yields with 30 μl of elution buffer. Lower amounts can be used but overall yield will be reduced due to incomplete wetting of the membrane. Eluting with small volumes such as 10 μl will provide highly concentrated plasmid DNA, but recovery may only reach 60%. Higher elution volumes can also be used, but the eluted DNA will have a lower concentration.
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