HomeFAQsWhat cycling conditions should I use for NEBNext Ultra II Q5 Master Mix?
FAQ: What cycling conditions should I use for NEBNext Ultra II Q5 Master Mix?
We have optimized the cycling condition for robust, low-bias amplification of NGS libraries. We recommend an extension temperature of 65°C for this application. If using primers other than NEBNext primers, you may need to optimize annealing temperature.