HomeFAQsCan I use NEBNext Ultra II Q5 Master Mix to do simple end-point PCR experiments?
FAQ: Can I use NEBNext Ultra II Q5 Master Mix to do simple end-point PCR experiments?
Yes. We also offer another Q5 master mix, M0494, which is optimized for typical end-point PCR. If using NEBNext Ultra II Q5 Master Mix for typical end-point PCR, we recommend using the M0494 cycling conditions that has an extension temperature of 72°C. More information can be found here.