Fourth of July Shipping Notice: Orders placed on Friday, July 1, 2022, will be shipped on Tuesday, July 5, 2022, for delivery on Wednesday, July 6, 2022.
HomeFAQsCan I scale up the PCR reaction volume in order to increase library yields?
FAQ: Can I scale up the PCR reaction volume in order to increase library yields?
We recommend a PCR volume of 50μl. Simply scaling up the PCR reaction does not increase the yield. You may gradually increase the PCR cycle number and/or increase the primer concentration in a 50μl PCR reaction to achieve higher yields. Please note that it is common for libraries with yields of 2μg or higher tend to show an amplification bias.