FAQ: Which enzyme should I use with Isothermal Amplifiction Buffer II?

Isothermal Amplification Buffer II was developed for use with Bst 3.0 DNA Polymerase. It is a very similar buffer to Isothermal Amplification Buffer, but with an increased concentration of KCl for optimal Bst 3.0 reaction performance. Use with Bst and Bst 2.0 is not recommended.