HomeFAQsWhen should Bst 3.0 DNA Polymerase be the enzyme of choice?
FAQ: When should Bst 3.0 DNA Polymerase be the enzyme of choice?
Bst 3.0 DNA Polymerase displays high strand displacement activity. It fills a void between thermophilic and mesophilic polymerases. The temperature optimum of 60–72°C is higher than DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210) and lower than Vent DNA Polymerase (NEB# M0254), two other strand displacing polymerases. This gives researchers a wider range of reaction conditions to optimize strand displacement and primer annealing. This is useful in the design of sequencing strategies as well as isothermal amplification technologies, and Bst 3.0 is the fastest, most robust enzyme for isothermal methods such as LAMP. It displays increased tolerance to inhibitors from diagnostic samples, allowing more consistent reactions with less concern for cleanup. Bst 3.0 has high reverse transcriptase activity up to 72 °C, enabling single-enzyme RT-LAMP reactions and cDNA synthesis through difficult secondary structures.