FAQ: What is the difference between Bst DNA Polymerase, Large Fragment, Bst 2.0, and Bst 3.0 DNA Polymerase?

All three polymerases are moderately thermostable DNA polymerases with strand displacement activity and can perform isothermal amplification reactions such as LAMP. However, Bst 2.0 DNA Polymerase is an in silico designed homolog of Bst DNA Polymerase, Large Fragment engineered for improved amplification reaction properties. Bst 3.0 features further improvements in amplification speed, inhibitor tolerance, thermostability and dUTP incorporation. Bst 3.0 also displays significantly higher reverse transcriptase activity up to 72 °C and can be used for single-enzyme RT-LAMP reactions.