FAQ: Does Bst 3.0 DNA polymerase have reverse transcriptase activity?

Yes. To use for RT applications we recommend elevated magnesium concentrations (4–8 mM). For general RT-LAMP reactions we recommend a dedicated reverse transcriptase (WarmStart RTx, NEB #M0380) in addition to the DNA-dependent DNA polymerase, but when a single-enzyme system is desired Bst 3.0 is the enzyme of choice.