HomeFAQsHow can I determine if my NEB 10-beta cells are competent?
FAQ: How can I determine if my NEB 10-beta cells are competent?
The pUC19 control DNA included in the kit is provided in case the user would like to confirm the competency of the provided NEB 10-beta Competent E. coli cells. While this is not routinely required, the transformation efficiency can be independently confirmed by transforming 100 pg (2 μl of the 50 pg/μl stock) into 50 μl competent cells and following the normal transformation and plating protocols. The resulting transformation efficiency should be > 1 x 108 cfu/μg pUC19 DNA.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.