HomeFAQsDoes the library quant require a size correction?
FAQ: Does the library quant require a size correction?
Yes. The intercalating dye used in the kit binds DNA in proportion to the number of base pairs per double-stranded DNA product, so it is necessary to correct for product length by normalizing to the size of the DNA standard, 399 bp. All quants are simply multiplied by a correction factor, 399/library size. This is done automatically using our online calculator tool.