FAQ: Are 4 standards enough for a qPCR quantitation?

Yes. Although we recommend using 6 standards (100 pM – 0.001pM), the standard curves generated from 4 standards (10 pM – 0.01pM) are sufficient to give a reliable measurement of library concentration. Even if one standard is an outlier from the curve due to problems in setup, the other 3 can be used with confidence. The range of standard concentrations provided with the kit will cover all libraries prepared using standard methods and kits following the kit guidelines for making library dilutions.