neb.com will be undergoing maintenance beginning Friday, December 9th, at 5pm ET and will last through Saturday, December 10th, at 3pm ET. Online ordering will not be available during this time. We apologize in advance for any inconvenience this may cause.
HomeFAQsHow much should I dilute my library to run in the Quant Kit?
FAQ: How much should I dilute my library to run in the Quant Kit?
We recommend an initial 1:1,000 dilution of the library using the provided Library Dilution Buffer. For most library preps this will result in >10 pM library, so we recommend two more 1:10 serial dilutions resulting in final 1:10,000 and 1:100,000 dilutions. For new protocols and libraries, we recommend running both of these dilutions in the Quant Kit. With more established workflows or to maximize the number of libraries quantitated, it is often possible to use only the 1:100,000 dilution for quantitation. If custom dilutions are desired, please ensure that final diluted libraries fall within the range of the standards, 100 pM – 1 fM.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
