HomeFAQsHow much should I dilute my library to run in the Quant Kit?
FAQ: How much should I dilute my library to run in the Quant Kit?
We recommend an initial 1:1,000 dilution of the library using the
provided Library Dilution Buffer. For most library preps this will
result in >10 pM library, so we recommend two more 1:10 serial
dilutions resulting in final 1:10,000 and 1:100,000 dilutions. For new
protocols and libraries we recommend running both of these dilutions in
the Quant Kit. With more established workflows or to maximize the number
of libraries quantitated, it is often possible to use only the
1:100,000 dilution for quantitation. If custom dilutions are desired,
please ensure that final diluted libraries fall within the range of the
standards, 10 pM – 10 fM. If you need dilution buffer in addition to
what is provided in the kit, it can be purchased separately.