New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsHow can I separate a deglycosylated protein from the glycosidase?
FAQ: How can I separate a deglycosylated protein from the glycosidase?
Any specific affinity method that will selectively capture the protein of interest (i.e. protein G for immunoglobulins) will be appropriate. Size exclusion is not recommended, unless the size of the protein is very different from the glycosidase. Finally, some of the NEB glycosidases (Remove-iT™ PNGase F, Endo S, Endo D) have an affinity tag, to allow removal of the enzyme from the reaction mixture by capturing the tagged glycosidase with chitin beads.
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