New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsCan glycosidases be used in combination for extensive digestion?
FAQ: Can glycosidases be used in combination for extensive digestion?
Yes, combinations of glycosidases can be used in the same reaction mixture, for example the Protein Deglycosylation Mix II (NEB #P6044) is a combination of five glycosidases. Other combinations are possible, as long as the pH range allows a common reaction buffer. When exoglycosidases are added to a mix with endoglycosidases, it is possible that the released glycans will be trimmed by them, and therefore will not be recovered intact. Also, since pH changes might affect performance, more units or longer incubation time might be necessary when a given enzyme is acting in a non-typical buffer.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.