HomeFAQsCan glycosidases be used in combination for extensive digestion?
FAQ: Can glycosidases be used in combination for extensive digestion?
Yes, combinations of glycosidases can be used in the same reaction mixture, for example the Protein Deglycosylation Mix (#P6039S) is a combination of five glycosidases. Other combinations are possible, as long as the pH range allows a common reaction buffer (please refer to our Glycosidases Double Digest Chart). When exoglycosidases are added to a mix with endoglycosidases, it is possible that the released glycans will be trimmed by them, and therefore will not be recovered intact. Also, since pH changes might affect performance, more units or longer incubation time might be necessary when a given enzyme is acting in a non-typical buffer.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.