FAQ: HiScribe™ T7 ARCA mRNA Kit (with tailing) What is the difference between the HiScribe T7 ARCA mRNA Kit (NEB #E2065) and the HiScribe T7 ARCA mRNA Kit (with Tailing)(NEB #E2060)?

Both of the HiScribe T7 ARCA mRNA kits can be used to synthesize RNA by co-transcriptional capping with ARCA. The HiScribe T7 ARCA mRNA kits provide an easy solution for producing ARCA capped mRNAs. E2065 is intended for templates which have an encoded Poly(A) tail. For templates which do not have a encoded Poly(A) tails, #E2060 contains E. coli Poly(A) Polymerase for the addition of Poly(A) tails after transcription and capping.