HomeFAQsHow can I minimize PCR-generated errors in my amplicon inserts?
FAQ: How can I minimize PCR-generated errors in my amplicon inserts?
Use a high-fidelity DNA polymerase and avoid over-amplification. We recommend the use of Q5® High-Fidelity DNA Polymerase formulations for maximal high-fidelity (NEB #M0491, #M0493), which is also available in Master Mix format (NEB #M0492, #M0494). Also, use the minimum number of cycles required to generate the amount of DNA required for assembly; this is usually 20 cycles or less.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.