FAQ: Can I use α1-3,4,6 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?

Yes. We recommend double digesting α1-3,4,6 Galactosidase with other exoglycosidases using 1X GlycoBuffer 1 (50mM NaAcetate, pH 5.5, 5mM CaCl2). To achieve optimal galactosidase activity, supplement the reaction with rHSA. We recommend using 1X GlycoBuffer 2 (50 mM sodium phosphate, pH 7.5) for digestion of α1-3,4,6 Galactosidase with PNGase F and/or O-Glycosidase.