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HomeFAQsWill SP6 RNA Polymerase work on uncut plasmid DNA?
FAQ: Will SP6 RNA Polymerase work on uncut plasmid DNA?
Yes, but SP6 RNA Polymerase is an extremely processive enzyme and will continue to transcribe around a circular template multiple times without disassociating. The plasmid can be linearized with a restriction enzyme that leaves a blunt or 5' overhang downstream of the DNA of interest.