FAQ: What is the promoter sequence of T7 RNA Polymerase?

T7 Promoter
5′ TAATACGACTCACTATAG 3′

T7 RNA polymerase starts transcription at the underlined G in the promoter sequence. The polymerase then transcribes using the opposite strand as a template from 5’->3’. The first base in the transcript will be a G.
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