FAQ: How can the yield of RNA be maximized when using T7 RNA Polymerase?

Higher yields of RNA may be obtained by raising NTP concentrations, amount of DNA template and amount of SP6 RNA Polymerase. NEB’s HiScribe T7 Kits have been optimized for synthesizing high yield unmodified RNA and capped and tailed mRNA. Please refer to the product pages for details.

E2040:HiScribe^®; T7 High Yield RNA Synthesis Kit

E2050:HiScribe^®; T7 Quick High Yield RNA Synthesis Kit

E2060: HiScribe® T7 ARCA mRNA Kit (with tailing)

E2065: HiScribe® T7 ARCA mRNA Kit