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HomeFAQsCan I use SP6 RNA Polymerase to make high specific activity radiolabeled probes?
FAQ: Can I use SP6 RNA Polymerase to make high specific activity radiolabeled probes?
Yes, T7 RNA Polymerase, SP6 RNA Polymerase and T3 RNA Polymerase and the HiScribe T7 Kit (NEB #E2040) can be used to make RNA probes. Higher detection sensitivity of nucleic acid can be obtained using an RNA probe due to the greater stability of RNA/DNA hybrids than DNA/DNA hybrids.