HomeFAQsWill the FFPE DNA Repair Mix ligate my DNA fragments?
FAQ: Will the FFPE DNA Repair Mix ligate my DNA fragments?
The ligase present in the FFPE DNA Repair Mix is Taq DNA ligase (NEB #M0208). This ligase effectively seals nicks in DNA, but is not effective at ligating fragments at a mismatch or at ligating blunt ended DNA. In fact, we have never observed this enzyme to ligate blunt ended DNA.