HomeFAQsIf I had a DNA template with mutation sites (ie. 8-oxoguanine or deaminated cytosines) that are directly adjacent to each other on opposite strands would treatment with FFPE DNA Repair Mix cause a double strand nick/break?
FAQ: If I had a DNA template with mutation sites (ie. 8-oxoguanine or deaminated cytosines) that are directly adjacent to each other on opposite strands would treatment with FFPE DNA Repair Mix cause a double strand nick/break?
We have not tried testing the repair of adjacent mutation sites on opposite strands. In this scenario, if the two damaged bases are removed at the same time, then a double stranded break could occur resulting in fragmented DNA. If, on the other hand, one reaction is faster than the other, and they do not occur simultaneously, then the FFPE DNA Repair Mix should repair them.
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