FAQ: Can the BioLux Gassia Luciferase Assay Kit (E3300) be used to assay GLuc activity in GLuc-containing blood samples and GLuc samples containing high serum?

Yes. However, to analyze the data it is important to have the proper controls (GLuc assay solution alone; GLuc-containing blood sample alone or GLuc sample containing high serum alone, i.e., without adding the GLuc assay solution to the sample) in the assay in order to establish the background signal. The presence of serum tends to increase the background signal of the GLuc activity measurements.

Links to this resource