HomeFAQsWhat is the difference between T7 Express (NEB #C2566H/I) and BL21(DE3) (NEB #C2527H/I)?
FAQ: What is the difference between T7 Express (NEB #C2566H/I) and BL21(DE3) (NEB #C2527H/I)?
T7-Express has the T7 RNA polymerase gene inserted into the lac operon on the E. coli chromosome and is expressed under the control of the lac promoter. This configuration provides controlled induction of the polymerase and consequently, inducible control of transcription of genes downstream of the T7 promoter. This system provides potential advantages over strains such as BL21(DE3), that carry the T7 RNA polymerase on a lysogenic prophage. Although λDE3 is normally dormant in the host chromosome, the induction of the SOS cascade can occur as the result of expressing proteins that damage the E. coli chromosome, either directly or indirectly. This may lead to cell lysis.
Choose your country
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.