HomeFAQsWhich enzyme should be used to remove β1-4 linked Galactose residues from intact IgG?
FAQ: Which enzyme should be used to remove β1-4 linked Galactose residues from intact IgG?
β1-4 linked galactose residues can be difficult to remove from intact IgG under native conditions, and the activity varies greatly depending on the subtype of IgG. Extended incubations and increased units are usually needed to see substrate turnover. β1-4 Galactosidase S (NEB# P0745) is the preferred enzyme for this purpose as β1-4 Galactosidase (NEB# P0730) has very low activity on intact IgG. A protocol using up to 50*g of Rituximab (CHO derived IgG1) with 80 units (10μl) of β1-4 Galactosidase S (NEB# P0745) in 1X GlycoBuffer 1 in a total volume of 50μl, incubated for 24 hours at 37°C yields efficient removal of β1-4 galactose residues. However, similar conditions treating a murine IgG2a yields only ~70-80% removal of β1-4 galactose residues.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.