FAQ: Can the BioLux GLuc Assay Kit be used for measuring Renilla luciferase activity?

Yes. The substrate, coelenterazine, is oxidized in the light reaction catalyzed by Gaussia or Renilla luciferase. Therefore, the GLuc assay reagents can be used for measuring Renilla luciferase activity. However, we recommend that the cell lysate be prepared by using the Luciferase Cell Lysis Buffer (NEB #B3321), since Renilla activity assay requires lysing the cells.