FAQ: Can the BioLux Gaussia Luciferase Assay Kit be used to assay GLuc activity in GLuc-containing blood samples and GLuc samples containing high serum?

Yes. However, to analyze the data it is important to have the proper controls (GLuc assay solution alone; GLuc-containing blood sample alone; or GLuc sample containing high serum alone) in the assay, in order to establish the background signal. The presence of serum tends to increase the background signal of the GLuc activity measurements.