HomeFAQsAre there any differences between the requirements for 2–3 fragment assemblies versus 4+?
FAQ: Are there any differences between the requirements for 2–3 fragment assemblies versus 4+?
The major differences between the two are the length of overlapping sequences between the adjacent fragments and the incubation time of the assembly reaction. The 15 minute assembly reaction protocol is recommended for assembly of 2–3 fragments that are flanked by 15–20 nt overlaps. The 1 hour assembly protocol is recommended for the assembly of 4+ fragments, flanked by 20–30 nt overlaps.