HomeFAQsCan a protease inhibitor cocktail be used in a Rapid PNGase F reaction?
FAQ: Can a protease inhibitor cocktail be used in a Rapid PNGase F reaction?
Yes. Protease cocktails are compatible with the Rapid PNGase F reaction. The following protease inhibitors have been tested: Aprotinin (10 μg/ml final concentration), Benzamidine (1mM final concentration), Pepstatin (10 μg/ml final concentration), Leupeptin (1μM final concentration), EGTA (1 mM final concentration), EDTA (1 mM final concentration) and PMSF (1 mM final concentration). Please note that we do not recommend PMSF as it has the ability to modify basic residues on glycoprotein substrates.
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