FAQ: Are downstream analysis such as HPLC and Mass Spectrometry compatible with Rapid PNGase F?

Yes, to prepare samples for liquid chromatography and/or mass spectrometry, N-glycans can be isolated by conventional solid phase extraction methods such as C18 and graphitized carbon (PGC). Refer to the “Glycan SPE C18 and Graphitized Carbon Protocols”. In addition to C18 and PGC, hydrophilic interaction (HILIC) may also be used downstream. For a proteomics experiment, the target antibody can be prepared using micro-dialysis or micro-filtration (refer to the “Glycoproteomics: Buffer Exchange Protocols”).