FAQ: Is NEBNext® Q5 Hot Start HiFi PCR Master Mix the same as NEBNext® High-Fidelity 2X PCR Master Mix (M0541)?

No. Although both master mixes contain Q5 High-Fidelity DNA Polymerase, the master mix composition is different. NEBNext Q5 Hot Start HiFi PCR Master Mix has been specifically optimized for even more robust amplification of NGS libraries in the presence or absence of magnetic beads and its hot start nature makes it ideal for workflows involving automation.