HomeFAQsDo I need to make any changes to the cycling conditions for my NGS library amplification I have previously been using with Phusion High-Fidelity PCR Master Mix?
FAQ: Do I need to make any changes to the cycling conditions for my NGS library amplification I have previously been using with Phusion High-Fidelity PCR Master Mix?
Yes. Cycling conditions for NEBNext Q5 Hot Start HiFi PCR Master Mix differ from those recommended for Phusion High-Fidelity PCR Master. NEBNext Q5 Hot Start HiFi PCR Master Mix has been optimized for robust, low-bias amplification of NGS libraries. We recommend using an extension temperature of 65C for this application. Please consult the guidelines included in the NEBNext kit product manuals for more specifics.
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