FAQ: Is RTx WarmStart® active in other buffers?

Yes, RTx WarmStart is generally active in buffers with pH >7.5, 10–200 mM monovalent salt, and 2–20 mM Mg2+.

Activity in other NEB Buffers:
NEBuffer1.1: 10%
NEBuffer2.1: 75%
NEBuffer3.1: 75%
CutSmart® Buffer: 90%
ThermoPol™: 90%