FAQ: How active is RTx WarmStart® at other temperatures?

<40°C: 0%, due to WarmStart® aptamer
42–60°C: 100%
60–65°C: 75%, but for <10 minutes
>65°C: 0%, but will be active during temperature ramping

RTx performs direct RT-LAMP at 65°C due to sufficiently high activity during reaction warm-up, but its use in cDNA synthesis protocols is not recommended above 55°C.