HomeFAQsDoes this enzyme require denaturing conditions to act on glycoproteins?
FAQ: Does this enzyme require denaturing conditions to act on glycoproteins?
No, in general exoglycosidases can remove sugar residues from native (folded) glycoproteins (hydrophilic glycans face away from the protein backbone). However, the protein folding around a glycan site might affect enzyme accessibility. Exoglycosidase digestion of some proteins will require longer incubation times, or in some cases a mild extent of denaturation.
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