HomeFAQsHow does One-Step RT-PCR kit protocol work for NEB #E5315?
FAQ: How does One-Step RT-PCR kit protocol work for NEB #E5315?
The One-Step RT-PCR reactions allow both cDNA synthesis and PCR amplification
in the same reaction buffer. A thermocycler is programmed so that
cDNA synthesis takes place first (48°C for 15 minutes), then reverse transcriptase
is inactivated while Hot Start Taq Polymerase is activated (94°C for
1 minute), followed by 40 cycles of PCR amplification.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.