FAQ: Does NEB provide plasmids for gRNA cloning?
Oligonucleotide DNA templates containing a T7 promoter and encoding sgRNA can be used with the HiScribe T7 Quick High Yield RNA Synthesis Kit (E2050).
We recommend using the EnGen® sgRNA Synthesis Kit, S. pyogenes (E3322S), which utilizes target-specific DNA oligos designed by the user and provides a quick method for transcribing high yields of sgRNA in a single 30 minute reaction.