FAQ: My restriction enzyme used to work well in the old NEBuffer but the new Performance chart indicates it has lower activity even though the only difference is the addition of BSA and removal of DTT to the new buffers. Why?

Through extensive testing with the new buffer system using a ratio of one unit of enzyme per ug of substrate, we have found some instances where our previous buffer recommendations could be adjusted. Please use the information that is indicated in the updated NEB Performance/Activity Chart to determine activity in various NEBuffers. This information indicates the most accurate activity of NEB restriction enzymes in NEBuffers.