FAQ: What type of ends does SplintR Ligase join?

SplintR Ligase is extremely effective at sealing nicks between adjacent residues of DNA strands hybridized to RNA splints or bridges, with an efficiency often 10-100 times that seen for T4 DNA ligase. On a fully-DNA substrate, this nick-sealing activity is equivalent to other DNA ligases. It is not a good choice for molecular cloning workflows in which double-strand breaks need to be repaired (such as joining a vector and an insert).