HomeFAQsCan I use a different competent E. coli strain than the provided NEB 10-beta strain?
FAQ: Can I use a different competent E. coli strain than the provided NEB 10-beta strain?
While this cloning system works well with a variety of cell strains, it is important to use a cell strain with robust growth to maintain selection pressure towards plasmid constructs containing your insert. In addition to the provided NEB 10-beta Competent E. coli (Cloning Efficiency) in NEB PCR Cloning Kit (NEB #E1202), cell strains that work well with this cloning kit include NEB Turbo (NEB #C2984) and NEB Express (NEB #C2523) Competent E. coli. The 5-alpha strains are not recommended, as their slightly slower growth rate does not support strong background suppression.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.