FAQ: Are SHuffle strains temperature sensitive?

SHuffle express cells are sensitive to elevated temperatures and do no grow at 42º C. But at temperatures 37º C and below, both SHuffle express (E. coli B) and SHuffle (E. coli K12) cells are viable. We therefore suggest growing SHuffle cells at non-stressed temperatures of 30º C and induce expression of your protein at even lower temperatures
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    Tips for Successful Transformations with NEB® Competent Cells

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