Home FAQs What volume of DNA can be added into chemically competent cells?

FAQ: What volume of DNA can be added into chemically competent cells?

The volume of DNA to be added into competent cells does affect transformation efficiency. 1-5 µl of DNA (plasmid or ligation product) is recommended for 50 µl of competent cells. When transforming a Gibson Assembly product, add 1-2 µl of the reaction to 50 µl of competent cells.