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  • FAQ: Will rSAP work in restriction enzyme NEBuffers?

    rSAP is active in all restriction enzyme NEBuffers 1.1, 2.1, 3.1 as well as NEBuffers 1, 2, 3, 4 and NEBuffer for EcoRI. And can be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary.